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The key difference between the
2020-07-24
The key difference between the membrane zymography and classical measurements of enzyme activities in soil slurries is the enzyme-substrate accessibility. Classical enzyme assays maximize access of substrate to all potentially reactive enzyme sites by ensuring sample destruction and detachment follo
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Ubiquitination is a reversible posttranslational modificatio
2020-07-24
Ubiquitination is a reversible posttranslational modification. The removal of Ub is carried out by enzymes known as deubiquitinases (DUBs). The antagonistic role played by these enzymes in the Ub pathway regulates the function of the ubiquitinated proteins, while maintaining the free Ub pool in euka
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Models and theories The CPA equation of state is
2020-07-24
Models and theories The CPA equation of state is composed predominantly of two parts: physical item and the association item based on the thermodynamic perturbation theory [21], [22], [26], [27]. The physical item is used to represent intermolecular physical interaction, usually representing the SR
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After the policy of the current British
2020-07-24
After 1915, the policy of the (current) British Commonwealth countries (including Britain, Canada, Australia, India, New Zealand and South Africa) regarding the dead, was to ensure that as far as possible, whether identified or not, all recovered bodies would be given individual graves, with standar
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The use of recombinant ER and ER binding assays offers
2020-07-23
The use of recombinant ERα and ERβ binding assays offers a relatively inexpensive, rapid technique for screening compounds for potential direct BEC receptor modulatory activity and understanding the mechanisms of existing herbal medicines. For background on the protein production system used to gen
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br Materials and methods br Results
2020-07-23
Materials and methods Results and discussion Conclusion The glycyl endopeptidase from papaya latex was partitioned using aqueous two-phase (10%PEG 6000–10% (NH4)2SO4) in combination with ammonium sulphate precipitation (40–60% saturation). The partially purified glycyl endopeptidase showed
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br DDR receptor and MMP expression Type II
2020-07-23
DDR2 receptor and MMP-13 expression Type II collagen protein in articular cartilage is highly stable structure as its half-life is 117 years [43]. The chondrocytes have limited ability to synthesis type II collagen protein in the matrix of mature articular cartilage once the collagen gets disrupt
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Unlike previous studies examining COMT genotyping in healthy
2020-07-23
Unlike previous studies examining COMT genotyping in healthy controls seeking to explain impulsive behaviors, this study sought to examine COMT genotypes in a large sample of individuals with varying levels of gambling behavior to determine whether COMT Pirfenidone receptor was associated with diffe
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8-CPT-Cyclic AMP sodium salt This study was aimed to assess
2020-07-23
This study was aimed to assess a number of esterase activities, namely erythrocyte AChE, plasma BuChE and BeChE, and serum PON1 towards a number of substrates, as well as the major genetic polymorphisms of BCHE and PON1in a cohort of greenhouse workers exposed to low toxicity pesticides under an int
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annexin v br Conclusion In summary a novel and label
2020-07-23
Conclusion In summary, a novel and label-free fluorescence assay was developed for detection of Cu2+ and galactose oxidase using water-dispersible homogenous alloyed CdZnTeS QDs. The CdZnTeS QDs were firstly synthesized via a simple one-pot hydrothermal route using DMSA as the S source, surface l
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Studies using SERPINB deficient mice established the signifi
2020-07-23
Studies using SERPINB1-deficient mice established the significant role for SERPINB1 in protecting lung antimicrobial proteins from proteolysis during microbe infection and its regulatory role in sustaining the balance of neutrophil reserve [15]. Neutrophil serine proteases (NSPs) (elastase, cathepsi
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Our approach including the characterization of histone
2020-07-23
Our approach, including the characterization of histone modifications in scarce NK subsets, allowed us to identify super-enhancers, which associate with genes endowing a given cell type with its unique identity (Hnisz et al., 2013). Especially intriguing were SEs with distinct activities in NK subse
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Here we describe preparation of three stable conjugates
2020-07-23
Here, we describe preparation of three stable conjugates that are linked by either oxyester, disulfide, or isopeptide bonds (Fig. 10.1). Each of these conjugates depends upon the prior purification of E2 and ubiquitin proteins that have been engineered to favor specific linkages. For the oxyester- a
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OxLDL down regulates eNOS and up regulates iNOS thereby
2020-07-22
OxLDL down-regulates eNOS and up-regulates iNOS, thereby augmenting the formation of NO and protein S-nitrosylation in human endothelial Fungicidin receptor [26]. Importantly, iNOS-mediated S-nitrosylation plays an increasingly significant role in cardiovascular diseases [34]. For example, iNOS-med
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There are two types of DDRs DDR and
2020-07-22
There are two types of DDRs, DDR1 and DDR2, which are type I transmembrane RTKs characterized by an N-terminal extracellular discoidin domain containing a collagen binding site [8]. DDR1 expression is somewhat restricted to epithelial cells, while DDR2 is often expressed in cells of mesenchymal orig
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